145574

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 4720416..4720996 Replicon chromosome
Accession NZ_CP047218
Organism Sphingobium yanoikuyae strain YC-JY1

Toxin (Protein)


Gene name pemK Uniprot ID A0A177JUB3
Locus tag GS397_RS22340 Protein ID WP_063976428.1
Coordinates 4720416..4720742 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A177JVZ4
Locus tag GS397_RS22345 Protein ID WP_063976429.1
Coordinates 4720739..4720996 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS397_RS22315 4715770..4716483 + 714 WP_007686167.1 phospholipase A -
GS397_RS22320 4716531..4717268 - 738 WP_007686165.1 hypothetical protein -
GS397_RS22325 4717276..4719309 - 2034 Protein_4416 AAA family ATPase -
GS397_RS22330 4719371..4720135 + 765 WP_015449415.1 IS6-like element IS6100 family transposase -
GS397_RS22335 4720164..4720313 + 150 WP_159368110.1 type II toxin-antitoxin system RelE/ParE family toxin -
GS397_RS22340 4720416..4720742 - 327 WP_063976428.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GS397_RS22345 4720739..4720996 - 258 WP_063976429.1 hypothetical protein Antitoxin
GS397_RS22350 4721279..4721929 - 651 WP_159367693.1 hypothetical protein -
GS397_RS22355 4721930..4722460 + 531 WP_159368111.1 hypothetical protein -
GS397_RS22360 4722574..4722726 + 153 WP_201450235.1 DUF1491 family protein -
GS397_RS22365 4722790..4723392 - 603 WP_159367695.1 DUF1269 domain-containing protein -
GS397_RS22370 4723633..4724988 - 1356 WP_006473457.1 IS1380-like element IS1247 family transposase -
GS397_RS22375 4725130..4725828 - 699 WP_081260992.1 TetR/AcrR family transcriptional regulator; helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4705898..4751811 45913
- inside IScluster/Tn - - 4719371..4724988 5617


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11899.77 Da        Isoelectric Point: 10.0037

>T145574 WP_063976428.1 NZ_CP047218:c4720742-4720416 [Sphingobium yanoikuyae]
VKRGDVYMVDLEPTHGREQRGHRPVVIVSPDDFNRATGLPLIVPITNGGDFARRIGFAVPLSGTKTTGVVRCDQPRVLDL
KIRNGRKVETLPQAIMDEVLARTATLLE

Download         Length: 327 bp

>T145574 NZ_CP062406:2643719-2644081 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 8903.25 Da        Isoelectric Point: 4.9731

>AT145574 WP_063976429.1 NZ_CP047218:c4720996-4720739 [Sphingobium yanoikuyae]
MHTTNLRKVGGSVMLAVPPALLDVLHLAAGAKVGLAVDNGKLVVEPGARPRYTMAELLGASDYSQPQPPEEREWVDASAV
GGELL

Download         Length: 258 bp

>AT145574 NZ_CP062406:2643552-2643722 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A177JUB3

Antitoxin

Source ID Structure
AlphaFold DB A0A177JVZ4

References