Detailed information of TA system TA145568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 8148..8728
• Replicon: plasmid unnamed4
• Accession: NZ_CP047196
• Organism: Klebsiella pneumoniae strain Kp36

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: GS419_RS29555
• Protein ID: WP_071177730.1
• Coordinates: 8414..8728 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: GS419_RS29550
• Protein ID: WP_000093040.1
• Coordinates: 8148..8426 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS419_RS29530	4593..6076	-	1484	Protein_4	group II intron reverse transcriptase/maturase	-
GS419_RS29535	6721..6966	+	246	WP_032440458.1	hypothetical protein	-
GS419_RS29540	7240..7611	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
GS419_RS29545	7608..7814	+	207	Protein_7	TonB family protein	-
GS419_RS29550	8148..8426	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
GS419_RS29555	8414..8728	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GS419_RS29560	8892..9320	+	429	WP_001140599.1	hypothetical protein	-
GS419_RS29565	9346..9525	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
GS419_RS29570	9552..10082	-	531	WP_071177729.1	hypothetical protein	-
GS419_RS29575	10089..10820	-	732	WP_071177728.1	replication-relaxation family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12001	12001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T145568 WP_071177730.1 NZ_CP047196:8414-8728 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T145568 NZ_CP062406:1154585-1154773 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT145568 WP_000093040.1 NZ_CP047196:8148-8426 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT145568 NZ_CP062406:1154296-1154556 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1