Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35996..36639 Replicon plasmid unnamed2
Accession NZ_CP047194
Organism Klebsiella pneumoniae strain Kp36

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag GS419_RS28315 Protein ID WP_001044770.1
Coordinates 36223..36639 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GS419_RS28310 Protein ID WP_001261282.1
Coordinates 35996..36226 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS419_RS28280 31219..31998 - 780 WP_013214009.1 site-specific integrase -
GS419_RS28285 32182..33186 - 1005 WP_011977814.1 hypothetical protein -
GS419_RS28290 33216..33419 - 204 WP_011977813.1 hypothetical protein -
GS419_RS28295 33465..33986 - 522 WP_013214008.1 hypothetical protein -
GS419_RS28300 34044..34436 - 393 WP_011977811.1 hypothetical protein -
GS419_RS28305 34473..35417 - 945 WP_011977810.1 hypothetical protein -
GS419_RS30235 35578..36039 - 462 WP_014343465.1 hypothetical protein -
GS419_RS28310 35996..36226 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GS419_RS28315 36223..36639 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
GS419_RS28320 36713..38275 + 1563 WP_004206609.1 AAA family ATPase -
GS419_RS28325 38260..39282 + 1023 WP_000361404.1 helicase UvrD -
GS419_RS28330 39538..40235 + 698 Protein_54 IS1 family transposase -
GS419_RS28335 40264..40536 + 273 Protein_55 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..142228 142228


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T145559 WP_001044770.1 NZ_CP047194:36223-36639 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T145559 NZ_CP062405:c1666138-1665986 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT145559 WP_001261282.1 NZ_CP047194:35996-36226 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT145559 NZ_CP062405:c1666762-1666163 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References