145558

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 204339..205009 Replicon plasmid unnamed1
Accession NZ_CP047193
Organism Klebsiella pneumoniae strain Kp36

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag GS419_RS27990 Protein ID WP_004213072.1
Coordinates 204566..205009 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag GS419_RS27985 Protein ID WP_004213073.1
Coordinates 204339..204569 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GS419_RS27960 199524..200303 - 780 WP_004213560.1 site-specific integrase -
GS419_RS27965 200300..201121 - 822 WP_004213562.1 hypothetical protein -
GS419_RS27970 202093..202299 + 207 WP_004213077.1 hypothetical protein -
GS419_RS27975 202289..202582 - 294 WP_004213076.1 hypothetical protein -
GS419_RS27980 202598..203731 - 1134 WP_004213075.1 DUF3800 domain-containing protein -
GS419_RS27985 204339..204569 + 231 WP_004213073.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GS419_RS27990 204566..205009 + 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
GS419_RS27995 205023..205439 - 417 Protein_219 restriction endonuclease -
GS419_RS28000 205443..205736 - 294 Protein_220 transposase -
GS419_RS30220 206153..206788 + 636 Protein_221 response regulator -
GS419_RS28010 207303..207709 - 407 Protein_222 GAF domain-containing protein -
GS419_RS28015 207800..208742 - 943 Protein_223 DUF1471 domain-containing protein -
GS419_RS28020 208791..209282 - 492 WP_011251327.1 DM13 domain-containing protein -
GS419_RS28025 209345..209620 - 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / rmpA2 / iutA / iucD / iucC / iucB / iucA 1..219800 219800


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T145558 WP_004213072.1 NZ_CP047193:204566-205009 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T145558 NZ_CP062405:1611535-1611630 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT145558 WP_004213073.1 NZ_CP047193:204339-204569 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT145558 NZ_CP062405:c1611715-1611658 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References