Detailed information of TA system TA145534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1389120..1389725
• Replicon: chromosome
• Accession: NZ_CP047171
• Organism: Xylella fastidiosa subsp. fastidiosa strain IVIA5235

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: D1605_RS06255
• Protein ID: WP_004089252.1
• Coordinates: 1389120..1389416 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D1605_RS06260
• Protein ID: WP_004089254.1
• Coordinates: 1389420..1389725 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1605_RS06230	1384285..1385262	+	978	WP_004087353.1	hypothetical protein	-
D1605_RS06235	1385521..1385967	+	447	WP_004087355.1	hypothetical protein	-
D1605_RS06240	1386071..1387315	-	1245	WP_011097983.1	phage tail protein	-
D1605_RS06245	1387319..1387879	-	561	WP_011097869.1	DUF2612 domain-containing protein	-
D1605_RS06250	1387876..1389021	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
D1605_RS06255	1389120..1389416	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1605_RS06260	1389420..1389725	+	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
D1605_RS06265	1389736..1390089	-	354	WP_011097986.1	hypothetical protein	-
D1605_RS06270	1390086..1390727	-	642	WP_187120434.1	hypothetical protein	-
D1605_RS06275	1390724..1391554	-	831	WP_004090766.1	hypothetical protein	-
D1605_RS06280	1391551..1391868	-	318	WP_011097873.1	hypothetical protein	-
D1605_RS06285	1391868..1392638	-	771	WP_004090769.1	hypothetical protein	-
D1605_RS06290	1392749..1392976	+	228	WP_004091377.1	hypothetical protein	-
D1605_RS06295	1392960..1393226	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1386071..1404370	18299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T145534 WP_004089252.1 NZ_CP047171:1389120-1389416 [Xylella fastidiosa subsp. fastidiosa]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T145534 NZ_CP062402:c2364633-2364367 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT145534 WP_004089254.1 NZ_CP047171:1389420-1389725 [Xylella fastidiosa subsp. fastidiosa]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT145534 NZ_CP062402:c2364884-2364633 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9