Detailed information of TA system TA145532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1216962..1217758
• Replicon: chromosome
• Accession: NZ_CP047171
• Organism: Xylella fastidiosa subsp. fastidiosa strain IVIA5235

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: Q87CQ8
• Locus tag: D1605_RS05320
• Protein ID: WP_004088112.1
• Coordinates: 1216962..1217573 (-)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: B2I551
• Locus tag: D1605_RS05325
• Protein ID: WP_004088114.1
• Coordinates: 1217570..1217758 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1605_RS05275	1212417..1213304	-	888	WP_012382597.1	YdaU family protein	-
D1605_RS05280	1213301..1214089	-	789	WP_011097897.1	Bro-N domain-containing protein	-
D1605_RS05285	1214086..1214718	-	633	WP_004088094.1	Bro-N domain-containing protein	-
D1605_RS05290	1214715..1214999	-	285	WP_004088096.1	hypothetical protein	-
D1605_RS05295	1214996..1215244	-	249	WP_011097898.1	hypothetical protein	-
D1605_RS05300	1215241..1215429	-	189	WP_004088102.1	hypothetical protein	-
D1605_RS05305	1215457..1215648	-	192	WP_004088104.1	hypothetical protein	-
D1605_RS05310	1215907..1216152	-	246	WP_004088108.1	hypothetical protein	-
D1605_RS05315	1216237..1216911	+	675	WP_012382600.1	helix-turn-helix transcriptional regulator	-
D1605_RS05320	1216962..1217573	-	612	WP_004088112.1	Fic/DOC family protein	Toxin
D1605_RS05325	1217570..1217758	-	189	WP_004088114.1	antitoxin VbhA family protein	Antitoxin
D1605_RS05330	1218150..1218686	+	537	WP_012382601.1	hypothetical protein	-
D1605_RS05335	1218683..1219096	+	414	WP_004088056.1	DUF1566 domain-containing protein	-
D1605_RS05340	1219093..1219494	+	402	WP_004088054.1	hypothetical protein	-
D1605_RS05345	1219491..1219640	+	150	WP_012382602.1	hypothetical protein	-
D1605_RS05350	1219726..1220004	+	279	WP_004088050.1	hypothetical protein	-
D1605_RS05355	1220027..1220218	+	192	WP_012382603.1	hypothetical protein	-
D1605_RS05360	1220239..1220583	+	345	WP_004088346.1	hypothetical protein	-
D1605_RS05365	1220623..1221444	+	822	WP_004088345.1	DUF2303 family protein	-
D1605_RS05370	1221460..1222386	+	927	WP_004088344.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1170909..1227508	56599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22832.60 Da
• Isoelectric Point: 5.1271

>T145532 WP_004088112.1 NZ_CP047171:c1217573-1216962 [Xylella fastidiosa subsp. fastidiosa]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGGLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T145532 NZ_CP062402:c2030358-2029996 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6837.77 Da
• Isoelectric Point: 4.9859

>AT145532 WP_004088114.1 NZ_CP047171:c1217758-1217570 [Xylella fastidiosa subsp. fastidiosa]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQIA

Nucleotide

• Length: 189 bp

>AT145532 NZ_CP062402:c2030525-2030355 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: Q87CQ8

Antitoxin

• AlphaFold DB: B2I551