Detailed information of TA system TA145526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 637524..638143
• Replicon: chromosome
• Accession: NZ_CP047165
• Organism: Pelistega ratti strain NLN63

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: F9B76_RS02620
• Protein ID: WP_159990692.1
• Coordinates: 637524..637820 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: F9B76_RS02625
• Protein ID: WP_201289371.1
• Coordinates: 637859..638143 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F9B76_RS02600	633025..634278	-	1254	WP_159990689.1	ATP-dependent Clp protease ATP-binding subunit ClpX	-
F9B76_RS02605	634376..635029	-	654	WP_159992064.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
F9B76_RS02610	635032..636336	-	1305	WP_159990690.1	trigger factor	-
F9B76_RS02615	636765..637220	+	456	WP_159990691.1	hypothetical protein	-
F9B76_RS02620	637524..637820	+	297	WP_159990692.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F9B76_RS02625	637859..638143	+	285	WP_201289371.1	putative addiction module antidote protein	Antitoxin
F9B76_RS02630	638311..639684	-	1374	WP_159990694.1	L-serine ammonia-lyase	-
F9B76_RS02635	639888..642641	+	2754	WP_159990695.1	bifunctional [glutamate--ammonia ligase]-adenylyl-L-tyrosine phosphorylase/[glutamate--ammonia-ligase] adenylyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11279.11 Da
• Isoelectric Point: 10.1823

>T145526 WP_159990692.1 NZ_CP047165:637524-637820 [Pelistega ratti]
MFKVIQHPEFSKWLNSIKDTVTKIRLKKRLDKAQRGNLGDVKAVGEGVYEMREFFGSGWRMYYIKRDDVLIIMLGGGDKN
TQSKDIQNAIELSKLLGD

Nucleotide

• Length: 297 bp

>T145526 NZ_CP062401:2695651-2695860 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10101.54 Da
• Isoelectric Point: 4.8041

>AT145526 WP_201289371.1 NZ_CP047165:637859-638143 [Pelistega ratti]
MAEELQTEEDIQVYINLVMEEDDPAELAHALGVIAKARGMTQIAKQAGIGRESLYKALREGANPRYGTIAKVLGALGLKL
VAVPANNTHQESLS

Nucleotide

• Length: 285 bp

>AT145526 NZ_CP062401:2695346-2695639 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA