Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73318..73961 Replicon plasmid pKP19-2029-KPC2
Accession NZ_CP047161
Organism Klebsiella pneumoniae strain KP19-2029

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag GSU00_RS27395 Protein ID WP_001044770.1
Coordinates 73318..73734 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GSU00_RS27400 Protein ID WP_001261282.1
Coordinates 73731..73961 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU00_RS27375 69422..69694 - 273 Protein_92 transposase -
GSU00_RS27380 69723..70419 - 697 Protein_93 IS1 family transposase -
GSU00_RS27385 70675..71697 - 1023 WP_000361404.1 helicase UvrD -
GSU00_RS27390 71682..73244 - 1563 WP_004206609.1 AAA family ATPase -
GSU00_RS27395 73318..73734 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
GSU00_RS27400 73731..73961 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GSU00_RS28470 73918..74379 + 462 WP_014343465.1 hypothetical protein -
GSU00_RS27405 74540..75484 + 945 WP_011977810.1 hypothetical protein -
GSU00_RS27410 75521..75913 + 393 WP_011977811.1 hypothetical protein -
GSU00_RS27415 75971..76492 + 522 WP_013214008.1 hypothetical protein -
GSU00_RS27420 76538..76741 + 204 WP_011977813.1 hypothetical protein -
GSU00_RS27425 76771..77775 + 1005 WP_011977814.1 hypothetical protein -
GSU00_RS27430 77959..78738 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / blaSHV-12 / blaKPC-2 - 1..127240 127240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T145522 WP_001044770.1 NZ_CP047161:c73734-73318 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T145522 NZ_CP062401:2322038-2322304 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT145522 WP_001261282.1 NZ_CP047161:c73961-73731 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT145522 NZ_CP062401:2321781-2322038 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References