Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 452958..453583 Replicon chromosome
Accession NZ_CP047160
Organism Klebsiella pneumoniae strain KP19-2029

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GSU00_RS02185 Protein ID WP_002882817.1
Coordinates 452958..453341 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GSU00_RS02190 Protein ID WP_004150355.1
Coordinates 453341..453583 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSU00_RS02170 450324..451226 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GSU00_RS02175 451223..451858 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GSU00_RS02180 451855..452784 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GSU00_RS02185 452958..453341 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GSU00_RS02190 453341..453583 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GSU00_RS02195 453788..454705 + 918 WP_002882812.1 alpha/beta hydrolase -
GSU00_RS02200 454719..455660 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GSU00_RS02205 455705..456142 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GSU00_RS02210 456139..456999 - 861 WP_002882807.1 virulence factor BrkB family protein -
GSU00_RS02215 456993..457592 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T145501 WP_002882817.1 NZ_CP047160:c453341-452958 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T145501 NZ_CP062398:c2425877-2425611 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT145501 WP_004150355.1 NZ_CP047160:c453583-453341 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT145501 NZ_CP062398:c2426134-2425877 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References