Detailed information of TA system TA145499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 81152..81822
• Replicon: chromosome
• Accession: NZ_CP047160
• Organism: Klebsiella pneumoniae strain KP19-2029

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: GSU00_RS00440
• Protein ID: WP_004213072.1
• Coordinates: 81152..81595 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: GSU00_RS00445
• Protein ID: WP_004213073.1
• Coordinates: 81592..81822 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSU00_RS00405	76564..76839	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
GSU00_RS00410	76902..77393	+	492	WP_011251327.1	DM13 domain-containing protein	-
GSU00_RS00415	77442..78362	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
GSU00_RS00420	78453..78859	+	407	Protein_80	GAF domain-containing protein	-
GSU00_RS27835	79374..80008	-	635	Protein_81	response regulator	-
GSU00_RS00430	80425..80718	+	294	Protein_82	transposase	-
GSU00_RS00435	80728..81138	+	411	WP_196976738.1	restriction endonuclease	-
GSU00_RS00440	81152..81595	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
GSU00_RS00445	81592..81822	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GSU00_RS00450	82430..83563	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
GSU00_RS00455	83579..83872	+	294	WP_004213076.1	hypothetical protein	-
GSU00_RS00460	83862..84068	-	207	WP_004213077.1	hypothetical protein	-
GSU00_RS00465	84420..84710	+	291	WP_004213078.1	hypothetical protein	-
GSU00_RS00470	84700..85599	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mph(E)	iucA / iucB / iucC / iucD / iutA / rmpA	18350..144695	126345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T145499 WP_004213072.1 NZ_CP047160:c81595-81152 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T145499 NZ_CP062398:c2052434-2052225 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT145499 WP_004213073.1 NZ_CP047160:c81822-81592 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT145499 NZ_CP062398:c2052739-2052446 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620