Detailed information of TA system TA145496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3536410..3537104
• Replicon: chromosome
• Accession: NZ_CP047158
• Organism: Anoxybacillus sp. PDR2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A160FBF3
• Locus tag: GRQ40_RS17055
• Protein ID: WP_044744379.1
• Coordinates: 3536410..3536796 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GRQ40_RS17060
• Protein ID: WP_159720487.1
• Coordinates: 3536796..3537104 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRQ40_RS17025	3532070..3533560	+	1491	WP_159720482.1	glycerol kinase GlpK	-
GRQ40_RS17030	3533746..3533898	-	153	WP_159720484.1	hypothetical protein	-
GRQ40_RS17035	3534006..3534644	+	639	WP_159720486.1	Bax inhibitor-1/YccA family protein	-
GRQ40_RS17040	3534753..3535629	+	877	Protein_3297	transposase	-
GRQ40_RS17045	3535719..3536035	-	317	Protein_3298	hypothetical protein	-
GRQ40_RS17050	3536032..3536367	-	336	Protein_3299	hypothetical protein	-
GRQ40_RS17055	3536410..3536796	-	387	WP_044744379.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GRQ40_RS17060	3536796..3537104	-	309	WP_159720487.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GRQ40_RS17065	3537567..3537851	-	285	WP_159720489.1	hypothetical protein	-
GRQ40_RS17070	3538304..3538783	+	480	WP_159720491.1	hypothetical protein	-
GRQ40_RS17075	3539239..3539612	+	374	Protein_3304	hypothetical protein	-
GRQ40_RS17080	3540078..3540273	-	196	Protein_3305	ATP-binding protein	-
GRQ40_RS17085	3540292..3540585	-	294	WP_066151042.1	helix-turn-helix domain-containing protein	-
GRQ40_RS17095	3541113..3541361	-	249	Protein_3307	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3536410..3558103	21693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-117)

Antitoxin

• Antitoxin PemI-like (24-100)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14359.37 Da
• Isoelectric Point: 4.6172

>T145496 WP_044744379.1 NZ_CP047158:c3536796-3536410 [Anoxybacillus sp. PDR2]
MPADVPERGDFVVLNFHPQAGHEQAGRRNAIVLSPKEFNQATGFIVVCPMTNQKKGYPFEVDLPKEGISLDGDGYPITGV
VLTDQIKSLDWKARNLTILKKYNPEDAQIEEIDRIIDECLAKIETYLT

Nucleotide

• Length: 387 bp

>T145496 NZ_CP062398:c798875-798687 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11596.23 Da
• Isoelectric Point: 5.7697

>AT145496 WP_159720487.1 NZ_CP047158:c3537104-3536796 [Anoxybacillus sp. PDR2]
MNAKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADQIGIKQGSEMELNMIENEGIITLKPKRTRKKYTLEELL
SQITPENRHEEIDFGVEGRELI

Nucleotide

• Length: 309 bp

>AT145496 NZ_CP062398:c799164-798904 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A160FBF3