145493

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1244993..1245910 Replicon chromosome
Accession NZ_CP047157
Organism Bacillus velezensis strain FJAT-45028

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag GRT15_RS06470 Protein ID WP_003154806.1
Coordinates 1245164..1245910 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag GRT15_RS06465 Protein ID WP_003154807.1
Coordinates 1244993..1245163 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRT15_RS06430 1240225..1241847 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
GRT15_RS06435 1241860..1242231 + 372 WP_014304856.1 hypothetical protein -
GRT15_RS06440 1242237..1242434 + 198 WP_044053582.1 XkdX family protein -
GRT15_RS06445 1242491..1243252 + 762 WP_044053129.1 hypothetical protein -
GRT15_RS06450 1243304..1243567 + 264 WP_003154815.1 hemolysin XhlA family protein -
GRT15_RS06455 1243581..1243844 + 264 WP_003154813.1 phage holin -
GRT15_RS06460 1243858..1244736 + 879 WP_024085195.1 N-acetylmuramoyl-L-alanine amidase -
GRT15_RS06465 1244993..1245163 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
GRT15_RS06470 1245164..1245910 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
GRT15_RS06475 1246014..1247012 - 999 WP_003154805.1 inorganic phosphate transporter -
GRT15_RS06480 1247025..1247642 - 618 WP_003154804.1 DUF47 domain-containing protein -
GRT15_RS06485 1247928..1249244 - 1317 WP_044053130.1 amino acid permease -
GRT15_RS06490 1249568..1250518 + 951 WP_014304860.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T145493 WP_003154806.1 NZ_CP047157:c1245910-1245164 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T145493 NZ_CP062395:2670816-2671178 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT145493 WP_003154807.1 NZ_CP047157:c1245163-1244993 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT145493 NZ_CP062395:2670649-2670819 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References