Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 477256..477893 Replicon chromosome
Accession NZ_CP047157
Organism Bacillus velezensis strain FJAT-45028

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GRT15_RS02460 Protein ID WP_003156187.1
Coordinates 477543..477893 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GRT15_RS02455 Protein ID WP_003156188.1
Coordinates 477256..477537 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRT15_RS02435 473621..474220 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
GRT15_RS02440 474313..474678 + 366 WP_060657770.1 holo-ACP synthase -
GRT15_RS02445 474843..475850 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
GRT15_RS02450 475967..477136 + 1170 WP_025853726.1 alanine racemase -
GRT15_RS02455 477256..477537 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GRT15_RS02460 477543..477893 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GRT15_RS02465 478011..478832 + 822 WP_003156182.1 STAS domain-containing protein -
GRT15_RS02470 478837..479202 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
GRT15_RS02475 479205..479606 + 402 WP_003156178.1 anti-sigma regulatory factor -
GRT15_RS02480 479618..480625 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
GRT15_RS02485 480689..481018 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
GRT15_RS02490 481015..481497 + 483 WP_003156173.1 anti-sigma B factor RsbW -
GRT15_RS02495 481463..482251 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GRT15_RS02500 482251..482853 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T145492 WP_003156187.1 NZ_CP047157:477543-477893 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T145492 NZ_CP062395:2651225-2651434 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACGCCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT145492 WP_003156188.1 NZ_CP047157:477256-477537 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT145492 NZ_CP062395:2650920-2651213 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAAGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References