Detailed information of TA system TA145491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3652172..3652794
• Replicon: chromosome
• Accession: NZ_CP047156
• Organism: Epidermidibacterium keratini strain EPI-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EK0264_RS17535
• Protein ID: WP_159547022.1
• Coordinates: 3652378..3652794 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EK0264_RS17530
• Protein ID: WP_159547021.1
• Coordinates: 3652172..3652381 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EK0264_RS17505	3647623..3648972	+	1350	WP_159547645.1	insulinase family protein	-
EK0264_RS17510	3649106..3649858	+	753	WP_192933049.1	4-hydroxy-tetrahydrodipicolinate reductase	-
EK0264_RS17515	3649863..3650756	-	894	WP_159547019.1	SDR family oxidoreductase	-
EK0264_RS17520	3650816..3651490	-	675	WP_159547647.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
EK0264_RS17525	3651513..3652091	-	579	WP_159547020.1	GNAT family N-acetyltransferase	-
EK0264_RS17530	3652172..3652381	+	210	WP_159547021.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EK0264_RS17535	3652378..3652794	+	417	WP_159547022.1	PIN domain nuclease	Toxin
EK0264_RS17540	3652878..3654080	+	1203	WP_159547648.1	winged helix DNA-binding domain-containing protein	-
EK0264_RS17545	3654070..3654351	-	282	WP_159547023.1	muconolactone Delta-isomerase	-
EK0264_RS17550	3654383..3655246	-	864	WP_159547024.1	catechol 1,2-dioxygenase	-
EK0264_RS17555	3655314..3656417	-	1104	WP_159547025.1	enolase	-
EK0264_RS17560	3656414..3657328	-	915	WP_159547026.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3524995..3683521	158526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-38)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14931.95 Da
• Isoelectric Point: 5.9781

>T145491 WP_159547022.1 NZ_CP047156:3652378-3652794 [Epidermidibacterium keratini]
VSALVDTSVWVEFLRGTQSAAADYVRHEVGRNLATCEPVMMELLAGLSAGDRTARVERMLLSQTWLRIDPHLDYRGAVDV
FHATRATGHAPRGLADCLIAAIALRAGVEVAHRDTDYEYIAAATGLATIDLRERTAAG

Nucleotide

• Length: 417 bp

>T145491 NZ_CP062395:2337209-2337475 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7784.89 Da
• Isoelectric Point: 4.1546

>AT145491 WP_159547021.1 NZ_CP047156:3652172-3652381 [Epidermidibacterium keratini]
MTRTNIEIDDALIAEVMDRFSLSTKREAVDFALRRLVGPVMTRQEMLMLEGSGWEGDLDELRGDPVTSV

Nucleotide

• Length: 210 bp

>AT145491 NZ_CP062395:2336958-2337209 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA