Detailed information of TA system TA145486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 152753..153425
• Replicon: chromosome
• Accession: NZ_CP047156
• Organism: Epidermidibacterium keratini strain EPI-7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EK0264_RS00735
• Protein ID: WP_159541982.1
• Coordinates: 152997..153425 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EK0264_RS00730
• Protein ID: WP_159541980.1
• Coordinates: 152753..153010 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EK0264_RS00715	149104..150171	-	1068	WP_159541974.1	phenylalanine--tRNA ligase subunit alpha	-
EK0264_RS00720	150258..151895	-	1638	WP_159541976.1	thiamine pyrophosphate-binding protein	-
EK0264_RS00725	151892..152668	-	777	WP_159541978.1	cyclase family protein	-
EK0264_RS00730	152753..153010	+	258	WP_159541980.1	DUF2191 domain-containing protein	Antitoxin
EK0264_RS00735	152997..153425	+	429	WP_159541982.1	type II toxin-antitoxin system VapC family toxin	Toxin
EK0264_RS00740	153431..154858	-	1428	WP_159541984.1	amidase	-
EK0264_RS00745	154998..156560	+	1563	WP_159541986.1	LLM class flavin-dependent oxidoreductase	-
EK0264_RS00750	156511..157944	+	1434	WP_159541988.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15607.81 Da
• Isoelectric Point: 6.6349

>T145486 WP_159541982.1 NZ_CP047156:152997-153425 [Epidermidibacterium keratini]
MLLADVNVFIHAHRPDTESSQEYAAWLTRALTGPEPFGVSSLVLSGFLRIVTNHRVFADPTPPDVALAFCEDVRSAPAAV
MTLPGPRHWQIFRQLCEATGARGNVVPDAYLAALAIEHGATWISTDTGFARFPGLRWRRPLD

Nucleotide

• Length: 429 bp

>T145486 NZ_CP062393:2695219-2695428 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9186.35 Da
• Isoelectric Point: 4.7694

>AT145486 WP_159541980.1 NZ_CP047156:152753-153010 [Epidermidibacterium keratini]
MRTTVNIDADLLRRAKELAARSDRSLGDVVDDALRVLLLRGETATPRAPVTLTQYGSGGLRPGVDLDNKDAMAELLDDDV
THAAR

Nucleotide

• Length: 258 bp

>AT145486 NZ_CP062393:2694914-2695207 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA