Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/YoeB-RelB
Location 2008652..2009181 Replicon chromosome
Accession NZ_CP047142
Organism Lactobacillus crispatus strain C25

Toxin (Protein)


Gene name Txe Uniprot ID A0A854ZB67
Locus tag GSR61_RS09940 Protein ID WP_025006274.1
Coordinates 2008652..2008909 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID V5E284
Locus tag GSR61_RS09945 Protein ID WP_013087009.1
Coordinates 2008906..2009181 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GSR61_RS09915 2003734..2004507 - 774 WP_065991111.1 tyrosine-protein phosphatase -
GSR61_RS09920 2004563..2006062 - 1500 WP_057727002.1 glycerol kinase GlpK -
GSR61_RS09925 2006187..2007002 + 816 WP_013087004.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
GSR61_RS09935 2007721..2008550 - 830 Protein_1905 helix-turn-helix transcriptional regulator -
GSR61_RS09940 2008652..2008909 - 258 WP_025006274.1 Txe/YoeB family addiction module toxin Toxin
GSR61_RS09945 2008906..2009181 - 276 WP_013087009.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GSR61_RS09950 2009277..2009408 - 132 WP_065991109.1 MFS transporter -
GSR61_RS09955 2009520..2009930 + 411 WP_065991107.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
GSR61_RS09960 2009936..2010229 + 294 WP_013087011.1 type II toxin-antitoxin system RelE/ParE family toxin -
GSR61_RS09965 2010299..2010985 + 687 WP_065991105.1 cobalamin biosynthesis protein CobQ -
GSR61_RS09970 2011030..2011515 - 486 WP_065991103.1 C40 family peptidase -
GSR61_RS09975 2011710..2012060 + 351 WP_065991101.1 ABC transporter substrate-binding protein -
GSR61_RS09980 2012458..2012739 + 282 WP_065991097.1 hypothetical protein -
GSR61_RS09985 2012760..2013689 - 930 WP_065991095.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10219.64 Da        Isoelectric Point: 8.9406

>T145484 WP_025006274.1 NZ_CP047142:c2008909-2008652 [Lactobacillus crispatus]
MIVAWTDDGWNDYVYWYDDGDYKKVSRINDLVKDMKRHPFTGIGKPEPLKRNLSGLWSRRIDSKNRIVYDCHKSMITIYS
CKDHY

Download         Length: 258 bp

>T145484 NZ_CP062393:2372703-2372969 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10597.82 Da        Isoelectric Point: 4.5716

>AT145484 WP_013087009.1 NZ_CP047142:c2009181-2008906 [Lactobacillus crispatus]
MDAVNYSNFRKNLKHYFKQVNDDSEPLIVTNKEPEDNVVVLGKEDYDALVETLEIQSNQYLMDKIERGRKQVSEGTIKQH
ELIDPEQVTKS

Download         Length: 276 bp

>AT145484 NZ_CP062393:2372452-2372703 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854ZB67

Antitoxin

Source ID Structure
AlphaFold DB V5E284

References