Detailed information of TA system TA145477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1056060..1056652
• Replicon: chromosome
• Accession: NZ_CP047134
• Organism: Xylella fastidiosa subsp. multiplex strain IVIA5901

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: DVT08_RS04660
• Protein ID: WP_004085590.1
• Coordinates: 1056060..1056356 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: DVT08_RS04665
• Protein ID: WP_004085592.1
• Coordinates: 1056359..1056652 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVT08_RS04635	1051229..1052197	+	969	WP_004084992.1	hypothetical protein	-
DVT08_RS04640	1052457..1052903	+	447	WP_004084994.1	hypothetical protein	-
DVT08_RS04645	1053007..1054251	-	1245	WP_187120419.1	phage tail protein	-
DVT08_RS04650	1054255..1054815	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
DVT08_RS04655	1054812..1055957	-	1146	WP_128283772.1	baseplate J/gp47 family protein	-
DVT08_RS04660	1056060..1056356	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DVT08_RS04665	1056359..1056652	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
DVT08_RS04670	1056708..1057061	-	354	WP_004084998.1	hypothetical protein	-
DVT08_RS04675	1057058..1057699	-	642	WP_012382583.1	hypothetical protein	-
DVT08_RS04680	1057696..1058526	-	831	WP_004085594.1	hypothetical protein	-
DVT08_RS04685	1058523..1058840	-	318	WP_004085001.1	hypothetical protein	-
DVT08_RS04690	1058840..1059610	-	771	WP_128283773.1	hypothetical protein	-
DVT08_RS04695	1059668..1059994	+	327	WP_004091353.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DVT08_RS04700	1059991..1060272	+	282	WP_004091355.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1053007..1097131	44124

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T145477 WP_004085590.1 NZ_CP047134:1056060-1056356 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T145477 NZ_CP062390:2752298-2752615 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTGATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTTGTTGAAGATAAATTTGTCAAAAATGAATTAGAAACAAAACAGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAGTTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGAATCAGATGACGCAAAGAAATTAAAAATTACAGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT145477 WP_004085592.1 NZ_CP047134:1056359-1056652 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT145477 NZ_CP062390:2752086-2752295 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTATCATGAATAATTTTGAAAGTTA
TGAACACACGGAATATGTTGATGGTATTGAAGTGGTTTCAGAGATCAGTCCTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAAAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: A0A060HCK8