145469

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4237877..4238515 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GRF62_RS20340 Protein ID WP_000813794.1
Coordinates 4238339..4238515 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GRF62_RS20335 Protein ID WP_001270286.1
Coordinates 4237877..4238293 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS20315 4233029..4233970 - 942 WP_001251304.1 ABC transporter permease -
GRF62_RS20320 4233971..4234984 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GRF62_RS20325 4235002..4236147 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GRF62_RS20330 4236392..4237798 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GRF62_RS20335 4237877..4238293 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GRF62_RS20340 4238339..4238515 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GRF62_RS20345 4238737..4238967 + 231 WP_000494244.1 YncJ family protein -
GRF62_RS20350 4239059..4241020 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GRF62_RS20355 4241093..4241629 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GRF62_RS20360 4241682..4242896 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T145469 WP_000813794.1 NZ_CP047127:c4238515-4238339 [Escherichia coli K-12]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T145469 NZ_CP062390:c166943-166848 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT145469 WP_001270286.1 NZ_CP047127:c4238293-4237877 [Escherichia coli K-12]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT145469 NZ_CP062390:166763-166820 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References