Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3669446..3670277 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag GRF62_RS17440 Protein ID WP_000854814.1
Coordinates 3669446..3669820 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag GRF62_RS17445 Protein ID WP_001285584.1
Coordinates 3669909..3670277 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS17405 3664842..3666008 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
GRF62_RS17410 3666127..3666600 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
GRF62_RS17415 3666798..3667856 + 1059 WP_046613327.1 FUSC family protein -
GRF62_RS17420 3668028..3668357 + 330 WP_000450409.1 DUF496 family protein -
GRF62_RS17425 3668458..3668640 - 183 WP_001016348.1 hypothetical protein -
GRF62_RS17430 3668712..3668840 + 129 Protein_3383 transposase domain-containing protein -
GRF62_RS22705 3669129..3669242 - 114 WP_001161660.1 DUF957 domain-containing protein -
GRF62_RS17435 3669255..3669449 - 195 WP_000988600.1 hypothetical protein -
GRF62_RS17440 3669446..3669820 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
GRF62_RS17445 3669909..3670277 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
GRF62_RS17450 3670351..3670572 - 222 WP_000692323.1 DUF987 domain-containing protein -
GRF62_RS17455 3670635..3671081 - 447 WP_000187523.1 RadC family protein -
GRF62_RS17460 3671078..3672610 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T145458 WP_000854814.1 NZ_CP047127:c3669820-3669446 [Escherichia coli K-12]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T145458 NZ_CP062388:123195-123347 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT145458 WP_001285584.1 NZ_CP047127:c3670277-3669909 [Escherichia coli K-12]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT145458 NZ_CP062388:122571-123170 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References