Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2712404..2713058 Replicon chromosome
Accession NZ_CP047127
Organism Escherichia coli K-12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GRF62_RS12990 Protein ID WP_000244777.1
Coordinates 2712651..2713058 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GRF62_RS12985 Protein ID WP_000354046.1
Coordinates 2712404..2712670 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRF62_RS12960 2707573..2708316 + 744 WP_000951964.1 SDR family oxidoreductase -
GRF62_RS12965 2708373..2709806 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
GRF62_RS12970 2709851..2710162 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GRF62_RS12975 2710326..2710985 + 660 WP_000250274.1 hemolysin III family protein -
GRF62_RS12980 2711181..2712161 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GRF62_RS12985 2712404..2712670 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GRF62_RS12990 2712651..2713058 + 408 WP_000244777.1 toxin CptA Toxin
GRF62_RS12995 2713098..2713619 - 522 WP_001055874.1 flavodoxin FldB -
GRF62_RS13000 2713731..2714627 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GRF62_RS13005 2714652..2715362 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GRF62_RS13010 2715368..2717101 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T145453 WP_000244777.1 NZ_CP047127:2712651-2713058 [Escherichia coli K-12]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T145453 NZ_CP062386:2592681-2592785 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT145453 WP_000354046.1 NZ_CP047127:2712404-2712670 [Escherichia coli K-12]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145453 NZ_CP062386:c2592943-2592780 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References