Detailed information of TA system TA145445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 1219073..1219887
• Replicon: chromosome
• Accession: NZ_CP047127
• Organism: Escherichia coli K-12

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GRF62_RS05865
• Protein ID: WP_001054376.1
• Coordinates: 1219073..1219330 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GRF62_RS05870
• Protein ID: WP_001309181.1
• Coordinates: 1219342..1219887 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRF62_RS05845	1214361..1215467	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
GRF62_RS05850	1215532..1216512	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GRF62_RS05855	1217095..1218335	-	1241	Protein_1144	DNA helicase	-
GRF62_RS05860	1218451..1218696	+	246	Protein_1145	GNAT family N-acetyltransferase	-
GRF62_RS05865	1219073..1219330	+	258	WP_001054376.1	hypothetical protein	Toxin
GRF62_RS05870	1219342..1219887	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GRF62_RS05875	1219943..1220689	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GRF62_RS05880	1220858..1221076	+	219	Protein_1149	hypothetical protein	-
GRF62_RS22670	1221114..1221230	+	117	Protein_1150	VOC family protein	-
GRF62_RS05885	1221475..1222596	+	1122	WP_000010829.1	M42 family metallopeptidase	-
GRF62_RS05890	1222593..1222871	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GRF62_RS05895	1222883..1224196	+	1314	WP_000460842.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	1208083..1228111	20028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T145445 WP_001054376.1 NZ_CP047127:1219073-1219330 [Escherichia coli K-12]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T145445 NZ_CP062384:2650309-2650671 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT145445 WP_001309181.1 NZ_CP047127:1219342-1219887 [Escherichia coli K-12]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT145445 NZ_CP062384:2650142-2650312 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8