Detailed information of TA system TA145440

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 844816..845495
• Replicon: chromosome
• Accession: NZ_CP047127
• Organism: Escherichia coli K-12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: GRF62_RS04115
• Protein ID: WP_000854672.1
• Coordinates: 845154..845495 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: GRF62_RS04110
• Protein ID: WP_000070395.1
• Coordinates: 844816..845133 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRF62_RS04060	839863..840726	+	864	WP_001065553.1	GTPase family protein	-
GRF62_RS04065	840818..841639	+	822	WP_000197389.1	DUF945 domain-containing protein	-
GRF62_RS04070	841856..842047	+	192	Protein_795	DeoR family transcriptional regulator	-
GRF62_RS04075	842043..842270	+	228	WP_001548158.1	hypothetical protein	-
GRF62_RS04080	842270..842713	+	444	WP_000824223.1	hypothetical protein	-
GRF62_RS04085	842736..843203	+	468	WP_001547765.1	hypothetical protein	-
GRF62_RS04090	843280..843519	+	240	WP_000194654.1	DUF905 domain-containing protein	-
GRF62_RS04095	843617..844075	+	459	WP_000211838.1	antirestriction protein	-
GRF62_RS04100	844091..844567	+	477	WP_000811693.1	RadC family protein	-
GRF62_RS04105	844576..844797	+	222	WP_000691994.1	DUF987 domain-containing protein	-
GRF62_RS04110	844816..845133	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GRF62_RS04115	845154..845495	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GRF62_RS04125	846067..847320	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
GRF62_RS04130	847332..848435	-	1104	WP_001285288.1	glutamate 5-kinase	-
GRF62_RS04135	848723..849082	+	360	Protein_807	porin	-
GRF62_RS04140	849189..850397	+	1209	WP_001352368.1	IS4-like element ISVsa5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T145440 WP_000854672.1 NZ_CP047127:845154-845495 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T145440 NZ_CP062384:121018-121170 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGAGAGTCAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT145440 WP_000070395.1 NZ_CP047127:844816-845133 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT145440 NZ_CP062384:120394-120993 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTAG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
CAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684