Detailed information of TA system TA145439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 726085..726703
• Replicon: chromosome
• Accession: NZ_CP047127
• Organism: Escherichia coli K-12

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: GRF62_RS03480
• Protein ID: WP_001290581.1
• Coordinates: 726485..726703 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: GRF62_RS03475
• Protein ID: WP_000344800.1
• Coordinates: 726085..726459 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GRF62_RS03465	721174..722367	+	1194	WP_001295324.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
GRF62_RS03470	722390..725539	+	3150	WP_001132469.1	multidrug efflux RND transporter permease subunit	-
GRF62_RS03475	726085..726459	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
GRF62_RS03480	726485..726703	+	219	WP_001290581.1	hemolysin expression modulator Hha	Toxin
GRF62_RS03485	726875..727426	+	552	WP_000102564.1	maltose O-acetyltransferase	-
GRF62_RS03490	727542..728012	+	471	WP_000136192.1	YlaC family protein	-
GRF62_RS03495	728176..729726	+	1551	WP_001310610.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
GRF62_RS03500	729768..730121	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
GRF62_RS03510	730500..730811	+	312	WP_000409911.1	MGMT family protein	-
GRF62_RS03515	730842..731414	-	573	WP_000779842.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8678.06 Da
• Isoelectric Point: 8.9008

>T145439 WP_001290581.1 NZ_CP047127:726485-726703 [Escherichia coli K-12]
MSEKFLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T145439 NZ_CP062384:20057-20233 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT145439 WP_000344800.1 NZ_CP047127:726085-726459 [Escherichia coli K-12]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT145439 NZ_CP062384:c20395-20221 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• AlphaFold DB: A0A7U9QBQ5