Detailed information of TA system TA145437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3154508..3155425
• Replicon: chromosome
• Accession: NZ_CP047119
• Organism: Bacillus velezensis strain AK-0

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GE573_RS16315
• Protein ID: WP_007407256.1
• Coordinates: 3154679..3155425 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GE573_RS16310
• Protein ID: WP_003154807.1
• Coordinates: 3154508..3154678 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE573_RS16275	3149729..3151360	+	1632	WP_127070286.1	pyocin knob domain-containing protein	-
GE573_RS16280	3151373..3151747	+	375	WP_190664217.1	hypothetical protein	-
GE573_RS16285	3151752..3151949	+	198	WP_059367156.1	XkdX family protein	-
GE573_RS16290	3152006..3152767	+	762	WP_190664218.1	phage portal protein	-
GE573_RS16295	3152819..3153082	+	264	WP_003154815.1	hemolysin XhlA family protein	-
GE573_RS16300	3153096..3153359	+	264	WP_190664219.1	phage holin	-
GE573_RS16305	3153373..3154251	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GE573_RS16310	3154508..3154678	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GE573_RS16315	3154679..3155425	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GE573_RS16320	3155530..3156528	-	999	WP_007407255.1	inorganic phosphate transporter	-
GE573_RS16325	3156541..3157158	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GE573_RS16330	3157444..3158760	-	1317	WP_007407254.1	amino acid permease	-
GE573_RS16335	3159083..3160033	+	951	WP_007407253.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T145437 WP_007407256.1 NZ_CP047119:c3155425-3154679 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T145437 NZ_CP062382:2769356-2769565 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACGCCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT145437 WP_003154807.1 NZ_CP047119:c3154678-3154508 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT145437 NZ_CP062382:2769051-2769344 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAAGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14