Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2392453..2393090 Replicon chromosome
Accession NZ_CP047119
Organism Bacillus velezensis strain AK-0

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GE573_RS12260 Protein ID WP_003156187.1
Coordinates 2392740..2393090 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GE573_RS12255 Protein ID WP_003156188.1
Coordinates 2392453..2392734 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GE573_RS12235 2388818..2389417 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
GE573_RS12240 2389510..2389875 + 366 WP_014304402.1 holo-ACP synthase -
GE573_RS12245 2390040..2391047 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
GE573_RS12250 2391164..2392333 + 1170 WP_156735897.1 alanine racemase -
GE573_RS12255 2392453..2392734 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GE573_RS12260 2392740..2393090 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GE573_RS12265 2393208..2394029 + 822 WP_003156182.1 STAS domain-containing protein -
GE573_RS12270 2394034..2394399 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
GE573_RS12275 2394402..2394803 + 402 WP_003156178.1 anti-sigma regulatory factor -
GE573_RS12280 2394815..2395822 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
GE573_RS12285 2395886..2396215 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
GE573_RS12290 2396212..2396694 + 483 WP_190664077.1 anti-sigma B factor RsbW -
GE573_RS12295 2396660..2397448 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GE573_RS12300 2397448..2398050 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T145436 WP_003156187.1 NZ_CP047119:2392740-2393090 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T145436 NZ_CP062382:2712107-2712211 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT145436 WP_003156188.1 NZ_CP047119:2392453-2392734 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT145436 NZ_CP062382:c2712370-2712206 [Staphylococcus aureus]
GTAGTAAGTAGAAGTAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References