Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 115655..116232 Replicon plasmid plas1.1.2
Accession NZ_CP047114
Organism Proteus mirabilis strain SCBX1.1

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag GO498_RS20290 Protein ID WP_023159957.1
Coordinates 115655..115987 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag GO498_RS20295 Protein ID WP_023159984.1
Coordinates 115987..116232 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GO498_RS20255 111239..112060 - 822 WP_001029679.1 TnsA endonuclease N-terminal domain-containing protein -
GO498_RS20260 112329..113282 - 954 WP_048821757.1 site-specific integrase -
GO498_RS20265 113576..113845 + 270 WP_048821758.1 hypothetical protein -
GO498_RS20270 113884..114138 + 255 WP_048821760.1 hypothetical protein -
GO498_RS20275 114237..114695 + 459 WP_048821762.1 hypothetical protein -
GO498_RS20280 115015..115155 + 141 WP_072022937.1 type I toxin-antitoxin system Hok family toxin -
GO498_RS20285 115244..115624 - 381 WP_048821763.1 hypothetical protein -
GO498_RS20290 115655..115987 - 333 WP_023159957.1 endoribonuclease MazF Toxin
GO498_RS20295 115987..116232 - 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GO498_RS20300 116525..117148 + 624 WP_023159958.1 AAA family ATPase -
GO498_RS20305 117265..117492 + 228 WP_048821767.1 hypothetical protein -
GO498_RS20310 117590..117958 - 369 WP_048821769.1 hypothetical protein -
GO498_RS20315 118719..119297 - 579 WP_048822182.1 hypothetical protein -
GO498_RS20320 119306..119785 - 480 WP_159242433.1 Hcp family type VI secretion system effector -
GO498_RS20325 120066..120458 - 393 WP_048821774.1 hypothetical protein -
GO498_RS20330 120468..120890 - 423 WP_159242432.1 hypothetical protein -
GO498_RS20335 120883..121227 - 345 WP_023159966.1 single-stranded DNA-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(F) / dfrA1 / ant(3'')-Ia / qacE / sul1 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / dfrA12 / aadA2 / qnrA1 / msr(E) / mph(E) / aph(3')-Ia / blaDHA-1 / ARR-3 / aac(6')-Ib-cr - 1..138818 138818


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T145434 WP_023159957.1 NZ_CP047114:c115987-115655 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T145434 NZ_CP062382:2382435-2382701 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT145434 WP_023159984.1 NZ_CP047114:c116232-115987 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT145434 NZ_CP062382:2382178-2382435 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References