Detailed information of TA system TA145432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3855091..3855790
• Replicon: chromosome
• Accession: NZ_CP047112
• Organism: Proteus mirabilis strain SCBX1.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: GO498_RS18085
• Protein ID: WP_004249093.1
• Coordinates: 3855091..3855477 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: GO498_RS18090
• Protein ID: WP_004246828.1
• Coordinates: 3855470..3855790 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GO498_RS18070	3851110..3851691	-	582	WP_004246833.1	DNA-3-methyladenine glycosylase I	-
GO498_RS18075	3851942..3852847	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
GO498_RS18080	3852857..3854929	+	2073	WP_041701373.1	glycine--tRNA ligase subunit beta	-
GO498_RS18085	3855091..3855477	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GO498_RS18090	3855470..3855790	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
GO498_RS18095	3855839..3856273	-	435	WP_004246827.1	hypothetical protein	-
GO498_RS18100	3856266..3857150	-	885	WP_004249091.1	endonuclease/exonuclease/phosphatase family protein	-
GO498_RS18105	3857367..3858653	-	1287	WP_049256516.1	DUF3748 domain-containing protein	-
GO498_RS18110	3858790..3859407	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
GO498_RS18115	3859709..3860332	+	624	WP_004246821.1	guanylate kinase	-
GO498_RS18120	3860387..3860662	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T145432 WP_004249093.1 NZ_CP047112:3855091-3855477 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T145432 NZ_CP062382:c1270522-1270271 [Staphylococcus aureus]
ATGGATAACCGTGAACAAATAGAACAATCCATTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTGCTAAA
AGAGATTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTTATCTTGATGAAGCAGTAGGGATTATGACGAGTCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT145432 WP_004246828.1 NZ_CP047112:3855470-3855790 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT145432 NZ_CP062382:c1270281-1270099 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATATACATTACAAGTTATCTGACGGCTCTATTATCAGTCTCATAGACTTTGTTATTAAACCAATTCATTTAATCA
AGGAGGCACAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97