Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 261079..261693 Replicon chromosome
Accession NZ_CP047073
Organism Pseudomonas syringae pv. tomato strain delta X

Toxin (Protein)


Gene name PfiT Uniprot ID Q88AZ2
Locus tag GRB21_RS01295 Protein ID WP_005768484.1
Coordinates 261343..261693 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID Q88AZ3
Locus tag GRB21_RS01290 Protein ID WP_007246542.1
Coordinates 261079..261330 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GRB21_RS01265 256145..256588 - 444 WP_172679536.1 ATP-dependent zinc protease -
GRB21_RS01270 256638..257420 - 783 WP_005768488.1 EAL domain-containing protein -
GRB21_RS01275 257588..257995 + 408 WP_003377907.1 RNA-binding protein S4 -
GRB21_RS01280 258156..259058 + 903 WP_005613652.1 Hsp33 family molecular chaperone HslO -
GRB21_RS01285 259238..260782 + 1545 WP_005613653.1 phosphoenolpyruvate carboxykinase -
GRB21_RS01290 261079..261330 + 252 WP_007246542.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GRB21_RS01295 261343..261693 + 351 WP_005768484.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GRB21_RS01300 261996..262448 - 453 WP_024666863.1 hypothetical protein -
GRB21_RS01305 262839..263453 - 615 WP_046463847.1 hypothetical protein -
GRB21_RS01310 263550..264257 - 708 WP_046463848.1 hypothetical protein -
GRB21_RS01315 264386..264835 - 450 WP_074843309.1 hypothetical protein -
GRB21_RS01320 265284..265997 - 714 WP_011103045.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-101)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13110.05 Da        Isoelectric Point: 4.3176

>T145411 WP_005768484.1 NZ_CP047073:261343-261693 [Pseudomonas syringae pv. tomato]
MNMFALRFTDVAQQSLEDQVEHLAVYQGFSPAAQRIDTLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNADGYRIFY
EVRETDDINVIVIVLVLGGKQSVEQALIRYCLLQPI

Download         Length: 351 bp

>T145411 NZ_CP062378:1558956-1559051 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9474.83 Da        Isoelectric Point: 9.5019

>AT145411 WP_007246542.1 NZ_CP047073:261079-261330 [Pseudomonas syringae pv. tomato]
MRVETISYLKRNAADLPLDEPLIVTQNGVPAYVVESYADRKRRDESIALVKLLAISSREYSQGKHCSADELKARLSRRFA
HKE

Download         Length: 252 bp

>AT145411 NZ_CP062378:c1559136-1559079 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88AZ2

Antitoxin

Source ID Structure
AlphaFold DB Q88AZ3

References