Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 20342..20934 Replicon plasmid unnamed
Accession NZ_CP047058
Organism Escherichia coli NC101

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag GQ584_RS24220 Protein ID WP_000520549.1
Coordinates 20342..20590 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag GQ584_RS24225 Protein ID WP_000681613.1
Coordinates 20587..20934 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS24175 (GQ584_23910) 15681..15932 + 252 WP_000121743.1 plasmid stabilization protein -
GQ584_RS24180 (GQ584_23915) 15922..16203 + 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin -
GQ584_RS24185 (GQ584_23920) 16227..16577 + 351 WP_000312755.1 hypothetical protein -
GQ584_RS24190 (GQ584_23925) 17217..17732 + 516 WP_001025397.1 J domain-containing protein -
GQ584_RS24195 (GQ584_23930) 17837..18379 + 543 WP_001083906.1 hypothetical protein -
GQ584_RS24200 (GQ584_23935) 18376..18627 + 252 WP_001230707.1 hypothetical protein -
GQ584_RS24205 (GQ584_23940) 18614..18841 + 228 WP_000801440.1 hypothetical protein -
GQ584_RS24210 (GQ584_23945) 19295..19594 + 300 WP_000650308.1 hypothetical protein -
GQ584_RS24215 (GQ584_23950) 19686..20036 + 351 WP_000854261.1 hypothetical protein -
GQ584_RS24220 (GQ584_23955) 20342..20590 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
GQ584_RS24225 (GQ584_23960) 20587..20934 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GQ584_RS24230 (GQ584_23965) 21298..21801 + 504 WP_000781818.1 DNA distortion polypeptide 1 -
GQ584_RS24235 (GQ584_23970) 21805..23034 + 1230 WP_024199200.1 DNA relaxase/nickase TaxC -
GQ584_RS24240 (GQ584_23975) 23111..23377 + 267 WP_000160396.1 hypothetical protein -
GQ584_RS24245 (GQ584_23980) 23382..23615 + 234 WP_000121162.1 EexN family lipoprotein -
GQ584_RS24250 (GQ584_23985) 24054..24674 + 621 WP_000157548.1 lytic transglycosylase domain-containing protein -
GQ584_RS24255 (GQ584_23990) 24688..25014 + 327 WP_000008647.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..45203 45203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T145390 WP_000520549.1 NZ_CP047058:20342-20590 [Escherichia coli NC101]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T145390 NZ_CP062370:2639966-2640175 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT145390 WP_000681613.1 NZ_CP047058:20587-20934 [Escherichia coli NC101]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT145390 NZ_CP062370:2639661-2639954 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References