Detailed information of TA system TA145388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 15681..16203
• Replicon: plasmid unnamed
• Accession: NZ_CP047058
• Organism: Escherichia coli NC101

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: GQ584_RS24180
• Protein ID: WP_000220561.1
• Coordinates: 15922..16203 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: GQ584_RS24175
• Protein ID: WP_000121743.1
• Coordinates: 15681..15932 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ584_RS24150 (GQ584_23885)	11013..11315	+	303	WP_001062963.1	hypothetical protein	-
GQ584_RS24155 (GQ584_23890)	11352..11603	+	252	WP_000905714.1	hypothetical protein	-
GQ584_RS24160 (GQ584_23895)	12175..12702	-	528	WP_000577047.1	DUF2726 domain-containing protein	-
GQ584_RS24165 (GQ584_23900)	12838..13242	-	405	WP_000654312.1	hypothetical protein	-
GQ584_RS24170 (GQ584_23905)	13308..14315	-	1008	WP_000243325.1	replication initiation protein	-
GQ584_RS24175 (GQ584_23910)	15681..15932	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
GQ584_RS24180 (GQ584_23915)	15922..16203	+	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQ584_RS24185 (GQ584_23920)	16227..16577	+	351	WP_000312755.1	hypothetical protein	-
GQ584_RS24190 (GQ584_23925)	17217..17732	+	516	WP_001025397.1	J domain-containing protein	-
GQ584_RS24195 (GQ584_23930)	17837..18379	+	543	WP_001083906.1	hypothetical protein	-
GQ584_RS24200 (GQ584_23935)	18376..18627	+	252	WP_001230707.1	hypothetical protein	-
GQ584_RS24205 (GQ584_23940)	18614..18841	+	228	WP_000801440.1	hypothetical protein	-
GQ584_RS24210 (GQ584_23945)	19295..19594	+	300	WP_000650308.1	hypothetical protein	-
GQ584_RS24215 (GQ584_23950)	19686..20036	+	351	WP_000854261.1	hypothetical protein	-
GQ584_RS24220 (GQ584_23955)	20342..20590	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
GQ584_RS24225 (GQ584_23960)	20587..20934	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..45203	45203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11023.86 Da
• Isoelectric Point: 10.5938

>T145388 WP_000220561.1 NZ_CP047058:15922-16203 [Escherichia coli NC101]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T145388 NZ_CP062370:2318852-2319118 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT145388 WP_000121743.1 NZ_CP047058:15681-15932 [Escherichia coli NC101]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT145388 NZ_CP062370:2318601-2318852 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301