Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4778535..4779032 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GQ584_RS22885 Protein ID WP_001173343.1
Coordinates 4778859..4779032 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag GQ584_RS22880 Protein ID WP_001312331.1
Coordinates 4778535..4778831 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS22860 (4775265) 4775265..4775984 - 720 WP_000611288.1 two-component system response regulator DcuR -
GQ584_RS22865 (4775981) 4775981..4777612 - 1632 WP_001216466.1 sensor histidine kinase -
GQ584_RS22870 (4777793) 4777793..4778023 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
GQ584_RS22875 (4778035) 4778035..4778307 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
GQ584_RS22880 (4778535) 4778535..4778831 + 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GQ584_RS22885 (4778859) 4778859..4779032 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GQ584_RS22890 (4779151) 4779151..4780668 - 1518 WP_000003805.1 lysine--tRNA ligase -
GQ584_RS22895 (4780905) 4780905..4782362 - 1458 WP_000856825.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T145387 WP_001173343.1 NZ_CP047057:4778859-4779032 [Escherichia coli NC101]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T145387 NZ_CP062370:2293576-2293683 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT145387 WP_001312331.1 NZ_CP047057:4778535-4778831 [Escherichia coli NC101]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT145387 NZ_CP062370:c2293760-2293700 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References