Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3275195..3275849 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GQ584_RS15675 Protein ID WP_000244781.1
Coordinates 3275195..3275602 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GQ584_RS15680 Protein ID WP_000354046.1
Coordinates 3275583..3275849 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS15655 (3271152) 3271152..3272885 - 1734 WP_000813193.1 single-stranded-DNA-specific exonuclease RecJ -
GQ584_RS15660 (3272891) 3272891..3273601 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GQ584_RS15665 (3273626) 3273626..3274522 - 897 WP_000806633.1 site-specific tyrosine recombinase XerD -
GQ584_RS15670 (3274634) 3274634..3275155 + 522 WP_001055874.1 flavodoxin FldB -
GQ584_RS15675 (3275195) 3275195..3275602 - 408 WP_000244781.1 protein YgfX Toxin
GQ584_RS15680 (3275583) 3275583..3275849 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GQ584_RS15685 (3276092) 3276092..3277072 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
GQ584_RS15690 (3277149) 3277149..3277808 - 660 WP_000250274.1 hemolysin III family protein -
GQ584_RS15695 (3277972) 3277972..3278283 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
GQ584_RS15700 (3278328) 3278328..3279761 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T145383 WP_000244781.1 NZ_CP047057:c3275602-3275195 [Escherichia coli NC101]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T145383 NZ_CP062370:123136-123288 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT145383 WP_000354046.1 NZ_CP047057:c3275849-3275583 [Escherichia coli NC101]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145383 NZ_CP062370:122512-123111 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References