Detailed information of TA system TA145372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1795637..1797225
• Replicon: chromosome
• Accession: NZ_CP047057
• Organism: Escherichia coli NC101

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A192ENQ5
• Locus tag: GQ584_RS08845
• Protein ID: WP_001125432.1
• Coordinates: 1795637..1796959 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: GQ584_RS08850
• Protein ID: WP_001304368.1
• Coordinates: 1796959..1797225 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ584_RS08810 (1791404)	1791404..1791637	-	234	WP_000473544.1	hypothetical protein	-
GQ584_RS08815 (1791672)	1791672..1791923	-	252	WP_000381239.1	membrane protein	-
GQ584_RS08820 (1791926)	1791926..1792243	-	318	WP_000073835.1	DNA-binding protein	-
GQ584_RS08825 (1792255)	1792255..1793307	-	1053	WP_000568109.1	phage/plasmid replication protein, II/X family	-
GQ584_RS08830 (1793479)	1793479..1793925	+	447	WP_000527682.1	helix-turn-helix transcriptional regulator	-
GQ584_RS08835 (1794025)	1794025..1794444	+	420	WP_000089315.1	hypothetical protein	-
GQ584_RS08840 (1794531)	1794531..1795460	+	930	WP_000963948.1	hypothetical protein	-
GQ584_RS08845 (1795637)	1795637..1796959	-	1323	WP_001125432.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GQ584_RS08850 (1796959)	1796959..1797225	-	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GQ584_RS08855 (1797533)	1797533..1798291	+	759	WP_001286543.1	trans-aconitate 2-methyltransferase	-
GQ584_RS08860 (1798295)	1798295..1799209	-	915	WP_001313828.1	bestrophin family protein	-
GQ584_RS08865 (1799406)	1799406..1800857	-	1452	WP_000854614.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1777801..1797243	19442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.02 Da
• Isoelectric Point: 8.6972

>T145372 WP_001125432.1 NZ_CP047057:c1796959-1795637 [Escherichia coli NC101]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T145372 NZ_CP062368:20153-20329 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT145372 WP_001304368.1 NZ_CP047057:c1797225-1796959 [Escherichia coli NC101]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT145372 NZ_CP062368:c20491-20317 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAGTGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• AlphaFold DB: A0A192ENQ5

Antitoxin

• AlphaFold DB: V0VAH2