Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 655348..655966 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GQ584_RS03145 Protein ID WP_001291435.1
Coordinates 655348..655566 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag GQ584_RS03150 Protein ID WP_000344800.1
Coordinates 655592..655966 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS03110 (650636) 650636..651208 + 573 WP_000779831.1 YbaY family lipoprotein -
GQ584_RS03115 (651238) 651238..651549 - 312 WP_000409908.1 MGMT family protein -
GQ584_RS03125 (651928) 651928..652281 + 354 WP_000878140.1 DUF1428 domain-containing protein -
GQ584_RS03130 (652323) 652323..653873 - 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GQ584_RS03135 (654037) 654037..654507 - 471 WP_000136192.1 YlaC family protein -
GQ584_RS03140 (654623) 654623..655174 - 552 WP_000102539.1 maltose O-acetyltransferase -
GQ584_RS03145 (655348) 655348..655566 - 219 WP_001291435.1 HHA domain-containing protein Toxin
GQ584_RS03150 (655592) 655592..655966 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
GQ584_RS03155 (656511) 656511..659660 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
GQ584_RS03160 (659683) 659683..660876 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T145369 WP_001291435.1 NZ_CP047057:c655566-655348 [Escherichia coli NC101]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T145369 NZ_CP062366:2767749-2767853 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT145369 WP_000344800.1 NZ_CP047057:c655966-655592 [Escherichia coli NC101]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT145369 NZ_CP062366:c2768012-2767848 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCATTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAAATGCCGGTCAAAGCGAATAGAAGGT
TATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References