Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 164483..165033 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag GQ584_RS00780 Protein ID WP_001160966.1
Coordinates 164719..165033 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag GQ584_RS00775 Protein ID WP_000125566.1
Coordinates 164483..164716 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS00750 (159556) 159556..159843 + 288 WP_000203741.1 ferredoxin-like protein FixX -
GQ584_RS00755 (159902) 159902..161233 + 1332 WP_001183207.1 MFS transporter -
GQ584_RS00760 (161341) 161341..161871 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GQ584_RS00765 (161864) 161864..163726 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
GQ584_RS00770 (163918) 163918..164397 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
GQ584_RS00775 (164483) 164483..164716 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GQ584_RS00780 (164719) 164719..165033 + 315 WP_001160966.1 CcdB family protein Toxin
GQ584_RS00785 (165030) 165030..165878 - 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GQ584_RS00790 (165885) 165885..166262 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GQ584_RS00795 (166265) 166265..167086 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GQ584_RS00800 (167083) 167083..168072 - 990 WP_000241228.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GQ584_RS00805 (168072) 168072..169358 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T145367 WP_001160966.1 NZ_CP047057:164719-165033 [Escherichia coli NC101]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T145367 NZ_CP062366:2476168-2476275 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT145367 WP_000125566.1 NZ_CP047057:164483-164716 [Escherichia coli NC101]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT145367 NZ_CP062366:c2476352-2476292 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References