Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 53999..54575 Replicon chromosome
Accession NZ_CP047057
Organism Escherichia coli NC101

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag GQ584_RS00235 Protein ID WP_001350372.1
Coordinates 53999..54286 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0G9FLJ7
Locus tag GQ584_RS00240 Protein ID WP_000063155.1
Coordinates 54273..54575 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ584_RS00220 (49632) 49632..50963 - 1332 WP_000505211.1 restriction endonuclease subunit S -
GQ584_RS00225 (50953) 50953..52572 - 1620 WP_001029741.1 class I SAM-dependent DNA methyltransferase -
GQ584_RS00230 (52636) 52636..53802 - 1167 WP_000800824.1 restriction endonuclease -
GQ584_RS00235 (53999) 53999..54286 + 288 WP_001350372.1 BrnT family toxin Toxin
GQ584_RS00240 (54273) 54273..54575 + 303 WP_000063155.1 BrnA antitoxin family protein Antitoxin
GQ584_RS00245 (54609) 54609..55565 - 957 WP_001350371.1 GTPase -
GQ584_RS00250 (55576) 55576..55779 - 204 WP_000467859.1 YbdD/YjiX family protein -
GQ584_RS00255 (55897) 55897..58047 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T145364 WP_001350372.1 NZ_CP047057:53999-54286 [Escherichia coli NC101]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T145364 NZ_CP062366:c222892-222797 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11572.05 Da        Isoelectric Point: 9.4646

>AT145364 WP_000063155.1 NZ_CP047057:54273-54575 [Escherichia coli NC101]
MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT145364 NZ_CP062366:222710-222769 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9FLJ7

References