Detailed information of TA system TA145344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2119391..2119920
• Replicon: chromosome
• Accession: NZ_CP047021
• Organism: Staphylococcus aureus strain M2024

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GQX63_RS10505
• Protein ID: WP_000621175.1
• Coordinates: 2119391..2119753 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GQX63_RS10510
• Protein ID: WP_000948331.1
• Coordinates: 2119750..2119920 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQX63_RS10485	2116369..2117139	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
GQX63_RS10490	2117114..2117593	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
GQX63_RS10495	2117595..2117921	-	327	WP_001052491.1	anti-sigma factor antagonist	-
GQX63_RS10500	2118040..2119041	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GQX63_RS10505	2119391..2119753	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GQX63_RS10510	2119750..2119920	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GQX63_RS10515	2120005..2121153	-	1149	WP_001281154.1	alanine racemase	-
GQX63_RS10520	2121219..2121578	-	360	WP_000581197.1	holo-ACP synthase	-
GQX63_RS10525	2121582..2122073	-	492	WP_072353916.1	PH domain-containing protein	-
GQX63_RS10530	2122060..2123643	-	1584	WP_045177766.1	PH domain-containing protein	-
GQX63_RS10535	2123636..2124115	-	480	WP_001287079.1	hypothetical protein	-
GQX63_RS10540	2124324..2124884	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T145344 WP_000621175.1 NZ_CP047021:c2119753-2119391 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T145344 NZ_CP062360:c2460248-2459982 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT145344 WP_000948331.1 NZ_CP047021:c2119920-2119750 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT145344 NZ_CP062360:c2460505-2460248 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1