Detailed information of TA system TA145331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 32046..32568
• Replicon: plasmid pX4
• Accession: NZ_CP047015
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GQF95_RS25575
• Protein ID: WP_113589067.1
• Coordinates: 32287..32568 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: GQF95_RS25570
• Protein ID: WP_000121743.1
• Coordinates: 32046..32297 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS25550 (GQF95_25490)	28542..29069	-	528	WP_000577047.1	DUF2726 domain-containing protein	-
GQF95_RS25555 (GQF95_25495)	29205..29609	-	405	WP_001541973.1	hypothetical protein	-
GQF95_RS25560 (GQF95_25500)	29673..30680	-	1008	WP_078405658.1	replication initiation protein	-
GQF95_RS26145	31692..31991	-	300	WP_251366424.1	hypothetical protein	-
GQF95_RS25570 (GQF95_25510)	32046..32297	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
GQF95_RS25575 (GQF95_25515)	32287..32568	+	282	WP_113589067.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GQF95_RS25580 (GQF95_25520)	32592..32942	+	351	WP_113589068.1	DNA-binding protein	-
GQF95_RS25585 (GQF95_25525)	33582..34097	+	516	WP_001025396.1	J domain-containing protein	-
GQF95_RS25590 (GQF95_25530)	34202..34744	+	543	WP_001083908.1	hypothetical protein	-
GQF95_RS25595 (GQF95_25535)	34741..34992	+	252	WP_001230707.1	hypothetical protein	-
GQF95_RS25600 (GQF95_25540)	34979..35206	+	228	WP_000801440.1	hypothetical protein	-
GQF95_RS26150	35291..35959	+	669	WP_251366425.1	hypothetical protein	-
GQF95_RS25615 (GQF95_25555)	36052..36402	+	351	WP_000854261.1	hypothetical protein	-
GQF95_RS25620 (GQF95_25560)	36707..36955	+	249	WP_113589071.1	type II toxin-antitoxin system HicA family toxin	-
GQF95_RS25625 (GQF95_25565)	36952..37299	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46955	46955
	-	flank	IS/Tn	-	-	27979..28254	275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11034.84 Da
• Isoelectric Point: 10.4466

>T145331 WP_113589067.1 NZ_CP047015:32287-32568 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPHVPAARLHGHTDRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T145331 NZ_CP062358:2318660-2318926 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT145331 WP_000121743.1 NZ_CP047015:32046-32297 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT145331 NZ_CP062358:2318409-2318660 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A3S5I301