Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 139117..139760 Replicon plasmid pfloR
Accession NZ_CP047011
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag GQF95_RS23710 Protein ID WP_001034044.1
Coordinates 139344..139760 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag GQF95_RS23705 Protein ID WP_001261286.1
Coordinates 139117..139347 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS23690 (134254) 134254..134484 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
GQF95_RS23695 (134481) 134481..134897 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
GQF95_RS23700 (134942) 134942..138736 - 3795 WP_001144732.1 hypothetical protein -
GQF95_RS23705 (139117) 139117..139347 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GQF95_RS23710 (139344) 139344..139760 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
GQF95_RS23715 (139835) 139835..141400 + 1566 WP_001128474.1 AAA family ATPase -
GQF95_RS23720 (141385) 141385..142407 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / tet(A) / sul2 / floR / erm(42) / sitABCD iutA / iucD / iucC / iucB / iucA 1..161471 161471
- flank IS/Tn - - 142661..143164 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T145320 WP_001034044.1 NZ_CP047011:139344-139760 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T145320 NZ_CP062356:2309465-2309731 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT145320 WP_001261286.1 NZ_CP047011:139117-139347 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT145320 NZ_CP062356:2309214-2309465 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References