Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 134254..134897 Replicon plasmid pfloR
Accession NZ_CP047011
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag GQF95_RS23695 Protein ID WP_001034046.1
Coordinates 134481..134897 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag GQF95_RS23690 Protein ID WP_001261278.1
Coordinates 134254..134484 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS23660 (129764) 129764..130069 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
GQF95_RS23665 (130071) 130071..130289 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
GQF95_RS23670 (130857) 130857..131369 + 513 WP_000151784.1 hypothetical protein -
GQF95_RS23675 (131403) 131403..132536 - 1134 WP_001542067.1 DUF3800 domain-containing protein -
GQF95_RS23680 (132703) 132703..133476 - 774 WP_000905949.1 hypothetical protein -
GQF95_RS23685 (133489) 133489..133989 - 501 WP_000528931.1 HEPN family nuclease -
GQF95_RS23690 (134254) 134254..134484 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
GQF95_RS23695 (134481) 134481..134897 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
GQF95_RS23700 (134942) 134942..138736 - 3795 WP_001144732.1 hypothetical protein -
GQF95_RS23705 (139117) 139117..139347 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
GQF95_RS23710 (139344) 139344..139760 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / mph(A) / aac(3)-IId / blaTEM-1B / sul3 / aph(3')-Ia / tet(A) / sul2 / floR / erm(42) / sitABCD iutA / iucD / iucC / iucB / iucA 1..161471 161471


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T145319 WP_001034046.1 NZ_CP047011:134481-134897 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T145319 NZ_CP062356:2284175-2284282 [Staphylococcus aureus]
ATGTTCAATTTATTAATTAACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT145319 WP_001261278.1 NZ_CP047011:134254-134484 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT145319 NZ_CP062356:c2284359-2284299 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References