Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3217591..3218216 Replicon chromosome
Accession NZ_CP047010
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag GQF95_RS15690 Protein ID WP_000911331.1
Coordinates 3217591..3217989 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GQF95_RS15695 Protein ID WP_000450524.1
Coordinates 3217989..3218216 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS15670 (3213469) 3213469..3213669 + 201 WP_089631931.1 YpfN family protein -
GQF95_RS15675 (3213779) 3213779..3214477 - 699 WP_000679812.1 esterase -
GQF95_RS15680 (3214551) 3214551..3216566 - 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
GQF95_RS15685 (3216581) 3216581..3217444 - 864 WP_001267507.1 neutral zinc metallopeptidase -
GQF95_RS15690 (3217591) 3217591..3217989 - 399 WP_000911331.1 type II toxin-antitoxin system VapC family toxin Toxin
GQF95_RS15695 (3217989) 3217989..3218216 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GQF95_RS15700 (3218370) 3218370..3219083 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GQF95_RS15705 (3219296) 3219296..3220330 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GQF95_RS15710 (3220347) 3220347..3221225 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GQF95_RS15715 (3221371) 3221371..3221943 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GQF95_RS15720 (3221943) 3221943..3222413 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T145309 WP_000911331.1 NZ_CP047010:c3217989-3217591 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T145309 NZ_CP062354:2304368-2304475 [Staphylococcus aureus]
ATCTTCAATTTATTAATTGACATCATGACTTCAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCAAATTGGTTACGAAC
GCGCAGCAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT145309 WP_000450524.1 NZ_CP047010:c3218216-3217989 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT145309 NZ_CP062354:c2304552-2304492 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References