Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2011731..2012369 Replicon chromosome
Accession NZ_CP047010
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GQF95_RS09780 Protein ID WP_000813794.1
Coordinates 2011731..2011907 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GQF95_RS09785 Protein ID WP_001270286.1
Coordinates 2011953..2012369 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS09760 (2007350) 2007350..2008525 - 1176 WP_241212901.1 BenE family transporter YdcO -
GQF95_RS09765 (2008617) 2008617..2009153 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
GQF95_RS09770 (2009226) 2009226..2011187 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GQF95_RS09775 (2011279) 2011279..2011509 - 231 WP_000494244.1 YncJ family protein -
GQF95_RS09780 (2011731) 2011731..2011907 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GQF95_RS09785 (2011953) 2011953..2012369 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GQF95_RS09790 (2012448) 2012448..2013854 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
GQF95_RS09795 (2014099) 2014099..2015244 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GQF95_RS09800 (2015262) 2015262..2016275 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
GQF95_RS09805 (2016276) 2016276..2017217 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2006045..2007310 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T145297 WP_000813794.1 NZ_CP047010:2011731-2011907 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T145297 NZ_CP062352:2252891-2253157 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAGGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT145297 WP_001270286.1 NZ_CP047010:2011953-2012369 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT145297 NZ_CP062352:2252634-2252891 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References