Detailed information of TA system TA145291

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 857888..858708
• Replicon: chromosome
• Accession: NZ_CP047010
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: GQF95_RS04265
• Protein ID: WP_000854821.1
• Coordinates: 858331..858708 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GQF95_RS04260
• Protein ID: WP_176476891.1
• Coordinates: 857888..858241 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS04220 (853333)	853333..854166	+	834	WP_096194525.1	DUF932 domain-containing protein	-
GQF95_RS04225 (854368)	854368..855072	+	705	WP_096194523.1	WYL domain-containing protein	-
GQF95_RS04230 (855069)	855069..855682	+	614	Protein_826	hypothetical protein	-
GQF95_RS04235 (855771)	855771..856181	+	411	WP_086660578.1	hypothetical protein	-
GQF95_RS04240 (856259)	856259..856495	+	237	WP_096210922.1	DUF905 domain-containing protein	-
GQF95_RS04245 (856574)	856574..857032	+	459	WP_086660576.1	antirestriction protein	-
GQF95_RS04250 (857048)	857048..857524	+	477	WP_001186725.1	RadC family protein	-
GQF95_RS04255 (857587)	857587..857808	+	222	WP_001601167.1	DUF987 domain-containing protein	-
GQF95_RS04260 (857888)	857888..858241	+	354	WP_176476891.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GQF95_RS04265 (858331)	858331..858708	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
GQF95_RS04270 (858705)	858705..858902	+	198	Protein_834	DUF5983 family protein	-
GQF95_RS04275 (858930)	858930..859127	+	198	WP_023563519.1	DUF957 domain-containing protein	-
GQF95_RS04280 (859212)	859212..860060	+	849	WP_096195002.1	DUF4942 domain-containing protein	-
GQF95_RS04285 (860153)	860153..861472	-	1320	WP_000144692.1	site-specific integrase	-
GQF95_RS04290 (861810)	861810..862148	-	339	Protein_838	LysR substrate-binding domain-containing protein	-
GQF95_RS04295 (862199)	862199..863155	-	957	WP_000121346.1	molybdenum cofactor insertion chaperone PaoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T145291 WP_000854821.1 NZ_CP047010:858331-858708 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T145291 NZ_CP062350:2634880-2635089 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTAATTTAG

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13152.89 Da
• Isoelectric Point: 6.4670

>AT145291 WP_176476891.1 NZ_CP047010:857888-858241 [Escherichia coli]
VSDTLPDDNHDRPWWGLPCTVTRCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLVKQLELMLTSSELNPH
RQNTVTLYVKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 354 bp

>AT145291 NZ_CP062350:2634575-2634868 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCGATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54