Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 314435..314932 Replicon chromosome
Accession NZ_CP047010
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GQF95_RS01605 Protein ID WP_001173343.1
Coordinates 314759..314932 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GQF95_RS01600 Protein ID WP_000398619.1
Coordinates 314435..314731 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS01580 (311166) 311166..311885 - 720 WP_000611288.1 two-component system response regulator DcuR -
GQF95_RS01585 (311882) 311882..313513 - 1632 WP_001216477.1 sensor histidine kinase -
GQF95_RS01590 (313694) 313694..313924 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
GQF95_RS01595 (313936) 313936..314208 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
GQF95_RS01600 (314435) 314435..314731 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GQF95_RS01605 (314759) 314759..314932 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GQF95_RS01610 (315051) 315051..316568 - 1518 WP_001295074.1 lysine--tRNA ligase -
GQF95_RS01615 (316805) 316805..318262 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T145288 WP_001173343.1 NZ_CP047010:314759-314932 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T145288 NZ_CP062350:2292652-2292759 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT145288 WP_000398619.1 NZ_CP047010:314435-314731 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT145288 NZ_CP062350:c2292836-2292776 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References