Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 36113..36911 Replicon plasmid pCTX
Accession NZ_CP047004
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag GQF94_RS23825 Protein ID WP_000072677.1
Coordinates 36390..36911 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag GQF94_RS23820 Protein ID WP_001351987.1
Coordinates 36113..36382 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS23790 (GQF94_23745) 31841..32581 - 741 WP_001717320.1 hypothetical protein -
GQF94_RS23795 (GQF94_23750) 32871..33476 + 606 WP_212040656.1 hypothetical protein -
GQF94_RS23805 (GQF94_23760) 34224..34706 + 483 Protein_36 hypothetical protein -
GQF94_RS23810 (GQF94_23765) 34767..35120 - 354 WP_160378290.1 hypothetical protein -
GQF94_RS23815 (GQF94_23770) 35126..35794 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
GQF94_RS23820 (GQF94_23775) 36113..36382 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
GQF94_RS23825 (GQF94_23780) 36390..36911 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
GQF94_RS23830 (GQF94_23785) 37080..37331 - 252 WP_000901559.1 hypothetical protein -
GQF94_RS23835 (GQF94_23790) 37333..38025 - 693 WP_012640731.1 hypothetical protein -
GQF94_RS23840 (GQF94_23795) 38039..38362 - 324 WP_000064175.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-55 - 1..112016 112016
- flank IS/Tn - - 33892..34167 275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T145285 WP_000072677.1 NZ_CP047004:36390-36911 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T145285 NZ_CP062350:c175552-175457 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT145285 WP_001351987.1 NZ_CP047004:36113-36382 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT145285 NZ_CP062350:175372-175429 [Staphylococcus aureus]
AACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References