Detailed information of TA system TA145276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4338841..4339678
• Replicon: chromosome
• Accession: NZ_CP047002
• Organism: Escherichia coli strain J-8

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GQF94_RS20685
• Protein ID: WP_000227784.1
• Coordinates: 4338841..4339383 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GQF94_RS20690
• Protein ID: WP_001297137.1
• Coordinates: 4339367..4339678 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF94_RS20665 (4334380)	4334380..4335291	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
GQF94_RS20670 (4335459)	4335459..4335950	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
GQF94_RS20675 (4336078)	4336078..4337442	-	1365	WP_001000978.1	MFS transporter	-
GQF94_RS20680 (4337850)	4337850..4338785	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
GQF94_RS20685 (4338841)	4338841..4339383	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GQF94_RS20690 (4339367)	4339367..4339678	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GQF94_RS20695 (4339863)	4339863..4340753	-	891	WP_000971332.1	heme o synthase	-
GQF94_RS20700 (4340765)	4340765..4341094	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GQF94_RS20705 (4341094)	4341094..4341708	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GQF94_RS20710 (4341698)	4341698..4343689	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GQF94_RS20715 (4343711)	4343711..4344658	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T145276 WP_000227784.1 NZ_CP047002:c4339383-4338841 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T145276 NZ_CP062348:c1853294-1852932 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT145276 WP_001297137.1 NZ_CP047002:c4339678-4339367 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT145276 NZ_CP062348:c1853461-1853291 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3