Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2758635..2759266 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag GQF94_RS13380 Protein ID WP_001259388.1
Coordinates 2758991..2759266 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GQF94_RS13375 Protein ID WP_000593555.1
Coordinates 2758635..2758994 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS13350 (2754767) 2754767..2755711 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
GQF94_RS13355 (2755708) 2755708..2756541 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
GQF94_RS13360 (2756541) 2756541..2757305 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
GQF94_RS13365 (2757302) 2757302..2758108 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
GQF94_RS13370 (2758114) 2758114..2758515 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
GQF94_RS13375 (2758635) 2758635..2758994 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GQF94_RS13380 (2758991) 2758991..2759266 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
GQF94_RS13385 (2759339) 2759339..2760463 - 1125 WP_001615305.1 ABC transporter permease -
GQF94_RS13390 (2760463) 2760463..2763198 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
GQF94_RS13395 (2763195) 2763195..2764262 - 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T145270 WP_001259388.1 NZ_CP047002:c2759266-2758991 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T145270 NZ_CP062346:2729333-2729695 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT145270 WP_000593555.1 NZ_CP047002:c2758994-2758635 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT145270 NZ_CP062346:2729166-2729336 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References