Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2129425..2130079 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GQF94_RS10230 Protein ID WP_000244772.1
Coordinates 2129425..2129832 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GQF94_RS10235 Protein ID WP_000354046.1
Coordinates 2129813..2130079 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS10210 (2125382) 2125382..2127115 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
GQF94_RS10215 (2127121) 2127121..2127831 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GQF94_RS10220 (2127856) 2127856..2128752 - 897 WP_000806637.1 site-specific tyrosine recombinase XerD -
GQF94_RS10225 (2128864) 2128864..2129385 + 522 WP_001055874.1 flavodoxin FldB -
GQF94_RS10230 (2129425) 2129425..2129832 - 408 WP_000244772.1 protein YgfX Toxin
GQF94_RS10235 (2129813) 2129813..2130079 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GQF94_RS10240 (2130322) 2130322..2131302 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
GQF94_RS10245 (2131498) 2131498..2132157 - 660 WP_000250274.1 hemolysin III family protein -
GQF94_RS10250 (2132321) 2132321..2132632 - 312 WP_001182945.1 N(4)-acetylcytidine aminohydrolase -
GQF94_RS10255 (2132677) 2132677..2134110 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
GQF94_RS10260 (2134167) 2134167..2134910 - 744 WP_000951941.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T145267 WP_000244772.1 NZ_CP047002:c2129832-2129425 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T145267 NZ_CP062346:2394279-2394545 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGCGCATTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT145267 WP_000354046.1 NZ_CP047002:c2130079-2129813 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145267 NZ_CP062346:2394028-2394279 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACATAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References