Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1700161..1700786 Replicon chromosome
Accession NZ_CP047002
Organism Escherichia coli strain J-8

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag GQF94_RS08205 Protein ID WP_000911329.1
Coordinates 1700161..1700559 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GQF94_RS08210 Protein ID WP_000450524.1
Coordinates 1700559..1700786 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF94_RS08185 (1696039) 1696039..1696239 + 201 WP_000383836.1 YpfN family protein -
GQF94_RS08190 (1696349) 1696349..1697047 - 699 WP_000679823.1 esterase -
GQF94_RS08195 (1697121) 1697121..1699136 - 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
GQF94_RS08200 (1699151) 1699151..1700014 - 864 WP_001267498.1 neutral zinc metallopeptidase -
GQF94_RS08205 (1700161) 1700161..1700559 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
GQF94_RS08210 (1700559) 1700559..1700786 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GQF94_RS08215 (1700941) 1700941..1701654 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GQF94_RS08220 (1701867) 1701867..1702901 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GQF94_RS08225 (1702918) 1702918..1703796 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GQF94_RS08230 (1703942) 1703942..1704514 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GQF94_RS08235 (1704514) 1704514..1704984 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T145265 WP_000911329.1 NZ_CP047002:c1700559-1700161 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T145265 NZ_CP062346:2337461-2337727 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTGATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT145265 WP_000450524.1 NZ_CP047002:c1700786-1700559 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT145265 NZ_CP062346:2337204-2337461 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References