Detailed information of TA system TA145254

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 534313..534951
• Replicon: chromosome
• Accession: NZ_CP047002
• Organism: Escherichia coli strain J-8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GQF94_RS02650
• Protein ID: WP_000813794.1
• Coordinates: 534313..534489 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GQF94_RS02655
• Protein ID: WP_001270285.1
• Coordinates: 534535..534951 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF94_RS02630 (529932)	529932..531107	-	1176	WP_001236265.1	BenE family transporter YdcO	-
GQF94_RS02635 (531199)	531199..531735	+	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
GQF94_RS02640 (531808)	531808..533769	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GQF94_RS02645 (533861)	533861..534091	-	231	WP_000494244.1	YncJ family protein	-
GQF94_RS02650 (534313)	534313..534489	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GQF94_RS02655 (534535)	534535..534951	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GQF94_RS02660 (535030)	535030..536436	+	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
GQF94_RS02665 (536681)	536681..537826	+	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
GQF94_RS02670 (537844)	537844..538857	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
GQF94_RS02675 (538858)	538858..539799	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	528744..529892	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T145254 WP_000813794.1 NZ_CP047002:534313-534489 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T145254 NZ_CP062344:c1229999-1229682 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGACATTGAAGTGATAAAACAAAAAATTAATGATGTAGCTACCAAACATGC
TTGGTTTGTTGAAGATAGATTTGTAAAAAATGAATTAGAAACAAAACGGGAACATATTAATTTTTCTGCTAGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACATGTGTACTTAAAAGAATTCGGTGAACTTATACAAAAATTT
CATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCACAAAAATTAAAAATCACAGAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT145254 WP_001270285.1 NZ_CP047002:534535-534951 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT145254 NZ_CP062344:c1230211-1230002 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAACTTGGAAAGTTA
TGAACACACAGAATATGTTAATAGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• PDB: 6HPB